Optimisation of methods for Agrobacterium rhizogenes mediated generation of composite plants in Eucalyptus camaldulensis

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Optimisation of methods for Agrobacterium rhizogenes mediated generation of composite plants in Eucalyptus camaldulensis

Background The wide adaptability, fast growth rate, good form, excellent wood and fiber properties make Eucalyptus the most widely planted genus of plantation forest trees. With the availability of Eucalyptus genome sequence information, assignment of function to genes contributing to desired traits require functional validation via overexpression or silencing in the context of the plant enviro...

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Wild-type Agrobacterium rhizogenes-mediated gene transfer in plants: Agrobacterium virulence and selection of transformants

Agrobacterium rhizogenes ATCC 15834 wild type strain was transformed with the binary vector pBI121 using the heat shock method. The transformed Agrobacterium was then tested for virulence through tobacco leaf explant transformation. Compared to the non-transformed Agrobacterium, the transformed Agrobacterium showed reduced virulence, producing signifi cantly lower number of hairy roots in tobac...

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Fertilization value of municipal sewage sludge for Eucalyptus camaldulensis plants

The wastewater treatment produces a large amount of sludge. The different uses of eliminations sludge such as landfills or incineration have consequences negative for the environment, the agricultural use has increased worldwide, especially in crops and few or no studies have been conducted with forest plantations in Algeria. The objective of this study is to assess fertilizing characteristics ...

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Agrobacterium Rhizogenes-mediated Transformation of Peganum multisectum (Maxim) Bobrov and Harmine Production in Hairy Roots

Using Agrobacterium rhizogenes due to create hairy roots is a useful method to product secondary metabolites in many medicinal plants. The transgenic hairy roots were induced from Peganum multisectum (Maxim) Bobrov a medicinally important species, by infecting leaf and stem explant with wild type Agrobacterium rhizogenes strain ATTCC 15834, which led to the induction of hairy roots from 19% of ...

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ژورنال

عنوان ژورنال: BMC Proceedings

سال: 2011

ISSN: 1753-6561

DOI: 10.1186/1753-6561-5-s7-o45